2/13/2023 0 Comments Stereology reference trap![]() ![]() However, the ability of a particular stereological probe to obtain accurate estimates is dependent on the spatial parameters selected for sampling, especially if the objects to be measured are not distributed uniformly throughout the prepared specimen. This approach allows valid comparisons to be made within and among studies, while avoiding the “reference trap” pitfall, in which the derived answer is based on the interplay of multiple variables (e.g., density is a function of both the number of objects and the volume they occupy). ![]() ![]() In contrast to two-dimensional image analysis, the goal of modern design-based stereology is to provide an unbiased estimate of the absolute number or size of measured objects within a three-dimensional region of interest, as opposed to a relative value (e.g., number of objects per unit area, i.e., density). ![]()
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